ABSOLUTE COUNTING OF PROTEINS SUSPENDED IN A THIN CELL USING HIGH RESOLUTION IMAGING TECHNOLOGY
The Occhio FC200SHR is part of a family of highly sophisticated imaging systems, that are byproducts of a series of collaborative studies in light, imaging, and sample transport, in connection with the University De Liege in Belgium. 

The FC200SHR IPAC, in particular, was developed to solve some of the current technological hurdles with protein aggregate counting. 
LIMITATIONS OF EXISTING TECHNOLOGY


HIGH RESOLUTION IMAGING TECHNOLOGY

This high resolution imaging technology, is accomplished in part, by using special backlighting techniques focused from behind the sample. An adjustable range, monochromatic, blue light source has been shown to provide optimal contrast conditions for the particles, and virtually eliminate diffraction. The light is collimated, and acts as a high definition caliper. (Fig. 1.2) In the case of liquids and suspensions, the light flashes simultaneously with a high speed, high definition camera, for extremely robust particle definitions.
COMPARISON SLIDES OF OPTICAL MICROSCOPY AND NEWLY DEVELOPED DIGITAL IMAGING TECHNIQUES
SUB MICRON DETECTION OF AGGREGATES-In order to improve the quality of the images, the Occhio FC200S HR uses a higher resolution camera, with an optical calibration of .38 microns per pixel, compared with an estimated 1.4 microns per pixel, on a Brightwell DPA 4200. The flow cell on an Occhio FC200S HR, is also half the width at 50 microns.
OCCHIO FLOWCELL
COMPARISON USING LATEX SPHERES
SPECIAL SYRINGE PUMP FOR PRECISE SAMPLING.
SOLUTIONS TO TECHNOLOGY LIMITATIONS

SUB-MICRONIC DETECTION OF AGGREGATES


LIQUID SUB SAMPLING

AGGREGATE DIFFERENTIAL SPEED
OCCHIO FC200SHR PARTICLE CHARACTERISTIC ANALYZER FOR PROTEIN AGGREGATES
SUB VISIBLE PARTICLE AND AGGREGATE DETECTION TO 200 nm
OCCHIO FLOWCELL TYPE PARTICLE ANALYZER FOR LIQUIDS AND SUSPENSIONS WITH SYRINGE PUMP AND HIGH RESOLUTION CAMERA OPTION FC200S HR

DYNAMIC ANALYSIS
APPLICATION
COLLIMATED LIGHT SOURCE ACTS LIKE A CALIPER TO PROVIDE SUPERIOR PARTICLE DEFINITION IN REAL TIME
IMAGE MAGNIFICATION IS INDEPENDENT OF THE OBJECTS DISTANCE OR POSITION IN THE FIELD OF VIEW
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OCCHIO FC200S IPAC FOR PROTEIN AGGREGATES
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AVAILABLE SIZE AND SHAPE PARAMETERS 
PARTICLE CHARACTERIZATION USING DIGITAL IMAGE ANALYSIS THEORY
SENSITIVITY OF PARTICLE SIZE ANDSHAPE PARAMETERS WITH RESPECT TO DIGITIZATION
DEMONSTRATION
WITH AUTO SAMPLER
DIGITAL IMAGING EQUIPMENT FOR PARTICLE CHARACTERIZATION IN LIQUIDS, POWDERS, AND  
                                 INSTALLATIONS WORLDWIDE   
HOMECONTACT USPRODUCTS

OCCHIO DIGITAL IMAGE ANALYZERS HAVE THE ABILITY TO DETECT AND QUANTIFY BOTH PARTICLE SIZE AND PARTICLE SHAPE, PAVING THE WAY FOR THE ADVANCED ANALYSIS OF PARTICLES IN BOTH LIQUID AND SOLID FORMS.

CORRELATIONS TO OTHER PARTICLE SIZE ANALYSIS METHODS, LIKE SIEVE ANALYSIS AND LASER DIFFRACTION, ARE SEAMLESSLY EMPLOYED. EVEN SHAPE PARAMETERS LIKE LENGTH, FLATNESS, AND ANGULARITY IN AGGREGATES, OR THE LENGTH OF STRINGY PARTICLES LIKE SHREDDED CHEESE, OR TOBACCO, ARE POSSIBLE.

ONCE A BASIC CORRELATION HAS BEEN ESTABLISHED, THE RESULTS ARE FINE TUNED WITH THE OCCHIO CORRELATION PROGRAM, ALLOWING FOR TURNKEY SOLUTIONS TO PARTICLE CHARACTERIZATION.
500 NANO SERIES500 NANO500 NANO PHARMA500 NANO XYCOFFEEE-TRAKFLOWCELL SERIES
FC200 IPACFC200MFC200SCMPSLURRYFC200SHRMORPHO 3-DPHARMA SCANNER
SIEVECALZEPHYR SERIESZEPHYR ESRZEPHYR LDAZEPHYR LDA COFFEE-TRAK

DIGITAL IMAGE ANALYZERS
DOWNLOADS
DIGITAL  PARTICLE
CHARACTERIZATION THEORY
EFFECTS OF DIGITIZATION
ON PARTICLE CHARACTERIZATION
AVAILABLE SIZE AND 
SHAPE PARAMETERS
SAMPLE 
CHARACTERISTICS

DISPERSION METHODS DESIGNED TO MAINTAIN PARTICLE INTEGRITY
GRANULAR MATERIALS, 
POWDERS, LIQUIDS

DRY GRANULAR MATERIALS 
SIZE RANGE 500 µm – 10,000 µm
EXAMPLES TABLETS, SEEDS, BEADS
3 DIMENSIONAL ANALYSIS 
DISPERSION METHOD
FEEDING HOPPER & CONVEYOR BELT
DRY POWDERS & GRANULARS
THAT TRAVEL WELL IN FLIGHT
7 µm – 5,000 µm 
EXAMPLES SUGAR / COFFEE / SAND
DISPERSION METHOD 
VIBRATING HOPPER WITH
HORIZONTAL VACUUM DISPERSION
 (FASTER ANALYSIS TIME)
LIQUID ANALYSIS FLOWCELL ACCESSORY
PARTICLES SUSPENDED IN LIQUID 1 µm – 3,000 µm
DRY GRANULARS HEAVIER PARTICLES 
30 µm – 30,000 µm
GRAVEL, WOOD CHIPS, SAND
COFFEE BEANS & GRINDS
DISPERSION METHOD
VIBRATING HOPPER WITH
VERTICAL DISPERSION (VACUUM ASSIST FOR DUST REMMOVAL)
LIQUID ANALYSIS FLOWCELL ACCESSORY
PARTICLES SUSPENDED IN LIQUID
30 µm – 1,000 µm 
FINE POWDERS
DRY 0.2 µm – 2,000 µm 
PHARMACEUTICALS, CHEMICALS
STATIC ANALYSIS
DISPERSION METHOD
PATENTED VACUUM IMPLOSION
LIQUID ANALYSIS FLOWCELL ACCESSORY
PARTICLES SUSPENDED IN LIQUID
.8 µm – 300 µm
DEDICATED LIQUID ANALYSIS
PARTICLES SUSPENDED IN LIQUID

​PARTICLES SUSPENDED IN LIQUID
0.8 µm-800 µm
DISPERSION METHOD
MEMBRANE PUMP (FOR PARTICLES SUBJECT 
TO BREAKAGE) 
PERILSTATIC PUMP (>500 µm)
PARTICLES SUSPENDED IN LIQUID
0.2 µm / 0.4 µm -1,000 µm
PROTEIN AGGREGATES
DISPERSION METHOD
SYRINGE PUMP
PARTICLES SUSPENDED IN LIQUID
SLURRIES
0.2 µm- 500 µm
DISPERSION METHOD
SYRINGE PUMP
SPECIAL DEDICATED 
APPLICATIONS
LABORATORY TEST SIEVE CALIBRATOR
CALIBRATE TEST SIEVES IN HOUSE.
2 MINUTES PER SIEVE.FULL INSPECTION / COMPLIANCE REPORT
PARTICLES IN LIQUIDS IN SITU
ANALYZE FIBERS, TEXTURES, AND PARTICLES INSIDE CONTAINERS